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Josiah Ramirez
Josiah Ramirez


The morphometric analysis was performed on five slides from the kidney of each animal. Quantitative data were obtained with a computerised system for image analysis NISElements Advanced Research (Ver. 2.30). The areas of interest in each slide were first found on low magnifications (x100, x200), taking into account the respective age group. Results were obtained through assessment of randomly selected zones of the renal cortex with no significant ruptures resulting from the processing technique of the histological material that could compromise the proper data analysis. The standardization of the zone where the areas of renal corpuscles and glomeruli were measured was performed in line with the following criteria in order to certify the authenticity of the results: 1. only renal corpuscles with clearly demarcated vascular and tubular poles were included in this study; 2. the selected renal corpuscles of the superficial nephrons were situated in the periphery of the cortex; 3. the analyzed renal corpuscles of the juxtamedullary nephrons were located adjacent to the medulla. The following morphometric parameters of the superficial and juxtamedullary nephrons during the postnatal development of SHR were analyzed: area of the renal corpuscles of the superficial nephrons (μm2), glomerular area of the superficial nephrons (μm2), area of the renal corpuscles of the juxtamedullary nephrons (μm2), glomerular area of the juxtamedulllary nephrons (μm2). The obtained quantitative data were demonstrated with Bar Chart/Bar Plot diagrams and were statistically evaluated through a Student-T-test. Statistically significant differences were read in the case of p

x100 ABV.BG.txt

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